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The cytotoxicity was measured using the index of lactate dehydrogenase (LDH) release from cells and expressed as a percentage of the total cellular activity. LuM1 cells were seeded at 5000 cells/well in 96-well NCP and cultured with or without Benz. The culture medium was transferred to the other 96-well plates at 24 h, 48 h, and 72 h after Benz addition. The LDH activity was measured using the LDH cytotoxicity assay kit according to the manufacturer’s instructions (Nacalai Tesque, Kyoto, Japan) by measuring the absorbance (490 nm).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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