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Secreted lactate was measured using the Lactate Colorimetric Assay Kit (catalog number MAK064-1KT; Sigma). Cells were plated in complete Dulbecco’s modified Eagle medium (RPMI interferes with the assay), allowed to adhere, washed with PBS, and left untreated or treated with LPS. Samples were collected at 1, 3, 6, and 24 hours, and the manufacturer’s protocol was followed to measure lactate.
Copyright and License information: ©2020 by the American Thoracic Society
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