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Intracellular chloride detection

JP Justin S. A. Perry
SM Sho Morioka
CM Christopher B. Medina
JE J. Iker Etchegaray
BB Brady Barron
MR Michael H. Raymond
CL Christopher D. Lucas
SO Suna Onengut-Gumuscu
ED Eric Delpire
KR Kodi S. Ravichandran
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In indicated efferocytosis assays, LR73 cells, peritoneal macrophages, or bone marrow-derived macrophages were labeled with N-[ethoxycarbonylmethyl]-6-methoxy-quinolinium bromide (MQAE) for 2 h in cell-type specific complete media. Cells were then washed and allowed to recover for 1 h in complete media prior to the start of the efferocytosis assay. MQAE signal emits optimally at 460nm and is easily detected by flow cytometry. Additionally, MQAE is not sensitive to pH, bicarbonate, borate, nitrate, or sulfate anions, making it suitable for detecting chloride changes regardless of other physiological cell changes 47.

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