4.7. Detection of Predicted miRNA/mRNA Targets

The list of 13 miRNAs upregulated during ATH progression in the 24 weeks old mice group treated with a scrambled SiRNA for 16 weeks (SC24W) was loaded onto the miRSystem browser (http://mirsystem.cgm.ntu.edu.tw), that uses seven different popular algorithms to predict miRNA targets [11], and the search was run with the default settings. All the potential target genes were copy-pasted onto an Excel spreadsheet, duplicated entries were eliminated as above, and the list was cleaned of all information but the official “GeneSymbol” and the “gene name”. This generated a list of 3658 predicted miRNA unique targets in Excel format (Hueso et al., 2019, In preparation). To identify miRNAs targeting the validated Oxstress genes, these were used to interrogate individually the miRSystem browser.