4.2. Synthesis of Amyloid Peptides

Aβ(1–42) and pGlu-Aβ(3–42) were synthesized by solid-phase synthesis and purified as described previously [67]. Structures and purities of Aβ peptides were confirmed by mass spectrometry. Before usage, peptides were dissolved in HFIP (Sigma-Aldrich, St. Louis, MO, USA) overnight. The solvents were evaporated under a stream of nitrogen and dissolved in 0.2 M NaOH, followed by buffer and finally titrated with 0.2 M HCl. Recombinant Aβ peptides were obtained as described previously [68].