2.10. Chromatin immunoprecipitation assays

Chromatin immunoprecipitation (ChIP) assays were performed as described in our previous publication (Wang et al., 2018). In brief, mouse liver samples were cross‐linked in 1% formaldehyde. The cross‐linked samples were digested with micrococcal nuclease and sonicated to reduce the DNA length (a final size of 150–900 bp). The sheared chromatin was incubated with anti‐CLOCK (Abcam, Cambridge, MA), anti‐REV‐ERBα (CST, Beverly, MA), or control rabbit IgG at 4°C overnight. Samples were de‐cross‐linked, and DNAs were purified, followed by qPCR analysis (the primers are listed in Table S3).