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Each HeLa cell sample or HeLa cell extract was incubated with 10 mM MβCD for 90 min at 37 °C under stirring. Then, the respective extracts were reconstituted in proteoliposomes.
Proteoliposomes reconstituted with the recombinant OCTN1 and added or not with CHS were incubated with MβCD as above described and then, MβCD was removed by chromatography on Sephadex G-75 column as described in Section 4.7.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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