RNA extraction and quantitative real-time PCR (qPCR)

EN Elin Nyman
SB Stefano Bartesaghi
RR Rebecka Melin Rydfalk
SE Sandra Eng
CP Charlotte Pollard
PG Peter Gennemark
XP Xiao-Rong Peng
GC Gunnar Cedersund
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Total RNA was extracted using RNAeasy (Qiagen) and reversed-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using TaqMan Gene Expression Assays (Supplementary Table S2). TBP served as loading controls.

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