5.4. Diabetic peripheral neuropathy (DPN) model

Rats were assessed for their baseline MWT nociceptive scores and then induced with type I diabetes with a single 55 mg/kg streptozocin i.v. injection and allowed to develop neuropathic pain. Once allodynia was confirmed with a von Frey assay 5–7 days after streptozocin injection, the rats were treated for 10 days with TPPU 3 mg/kg orally exactly as the naïve animals in previous experiments. The von Frey scores were measured to assess the treatment over 10 days. Thermal sensitivity was not assessed because it is subject to change over the time course in the diabetic neuropathy model due to c-fiber loss. For the graphs the daily pre- and 60 minute post-treatment scores are depicted to reveal the change from normal baseline in naïve animals or the painful neuropathic baseline compared to treatment in diabetic neuropathic animals.