Bacterial Minimum Inhibitory Concentration (MIC) Assay

The chemical stock solutions were diluted using a 2-fold serial dilution method.⁵⁶ Then, 10 μL of the diluted/undiluted stock solution was placed in a 96-well plate with 190 μL of fresh BHI-diluted L. monocytogenes EGDe overnight culture (1:1000 dilution). The plate was incubated in a microplate reader (BioTek Instruments, Inc., Winooski, VT) at 37 °C with 0.05 linear agitation. The absorbance at 600 nm (OD₆₀₀) was monitored by the microplate reader every hour for 24 h. The lowest chemical concentration among the groups that showed no increase of OD₆₀₀ over 24 h was determined as MIC.