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Live imaging of zebrafish embryos

AL Aaron P. van Loon
IE Ivan S. Erofeev
IM Ivan V. Maryshev
AG Andrew B. Goryachev
AS Alvaro Sagasti
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Live zebrafish embryos were anesthetized with 0.2 mg/ml MS-222 (Western Chemical) in system water before mounting. Embryos were embedded in 1.2% agarose on a coverslip and sealed within a microscope chamber, as previously described (O’Brien et al., 2009). Chambers were filled with 0.2 mg/ml MS-222 solution and sealed with vacuum grease. Images taken up to 32 hpf show the top of the head, and images taken after 32 hpf show the side of the trunk, avoiding the yolk extension.

Copyright and License information:  ©2020 van Loon et al.
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