Improve Research Reproducibility A Bio-protocol resource
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KE Karen L. Elliott
JK Jennifer Kersigo
NP Ning Pan
IJ Israt Jahan
BF Bernd Fritzsch
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Brains and ears were micro dissected, mounted on a slide with 100% glycerol as previously described (Fritzsch et al., 2016), and viewed using a Leica SP5 confocal microscope. Stacks of images were processed using Leica LIF software, combined into plates using Corel Draw. Aoth1-LacZ reactions were flat mounted in glycerol and imaged using a Nikon upright microscope with Metamorph software for image processing.

Copyright and License information:  ©2017 Elliott, Kersigo, Pan, Jahan and Fritzsch.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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