DNA extraction, amplification and sequencing

The genomic DNA extraction was performed as described by Limtong et al. [43]. The sequences of the gene encoding the D1/D2 region of the LSU rRNA gene and the internal transcribe spacer (ITS) region were amplified with the primers NL1 (5'-GCATATCAATAA GCGGGGAAAAG-3') and NL4 (5'-GGTCCGTGTTTCAAGACGG-3') [44] and the primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGA TATGC-3') [45], respectively. The PCR products were purified by using the TAINquick Midi Purification Kit (Tiangen Biotech, China) according to the manufacturer’s instructions. The purified products were sequenced by First Base Laboratories Sdn Bhd (Malaysia) using the PCR primers.