Virus culture

AC Agathe M. G. Colmant
HB Helle Bielefeldt-Ohmann
LV Laura J. Vet
CO Caitlin A. O’Brien
RB Richard A. Bowen
AH Airn E. Hartwig
SD Steven Davis
TP Thisun B. H. Piyasena
GH Gervais Habarugira
JH Jessica J. Harrison
JH Jody Hobson-Peters
RH Roy A. Hall
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The virus strains used were WNV, Kunjin strain MRM61C (WNVKUN) (passage unknown, Genbank accession number KX394398.1), WNVKUN strain WNVFLSDX(Pro) (derived from the WNVKUN infectious clone FLDSX–Genbank accession number AY274504.1, with a proline substitution in residue 250 of NS1 [43]) and BgV prototype CY4270 (passage 6, passaged only in C6/36 cells, Genbank accession number KU308380 for a passage 2 stock). The WNV-derived chimeric fragments were generated from WNVKUN infectious clone WNVFLSDX(Pro).

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