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An RT2 Profiler PCR Array [96-well format and 384-well (4 × 96) format, Cat. no. 330231, Qiagen] was applied for our analysis. A total of 10 µl of RT2 SYBR Green Mastermix, nuclease-free H2O and cDNA was added to each well. The cycling program included preincubation for 10 min at 95 °C (1 cycle), denaturation for 15 s at 95 °C, and annealing and extension for 1 min at 60 °C (40 cycles). Genes that changed significantly (p < 0.05) are presented in Fig. 6b. The original data are presented in Additional file 4: Table S1.
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