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MRC5 toxicity assay

FP Fabrice Pagniez
NL Nicolas Lebouvier
YN Young Min Na
IO Isabelle Ourliac-Garnier
CP Carine Picot
MB Marc Le Borgne
PP Patrice Le Pape
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Cytotoxicity of compounds was studied with human fibroblast (MRC5). Cells were grown in RPMI 1640 medium (Sigma-aldrich) supplemented with 10% foetal bovin serum (Sigma-aldrich). Drugs were tested at three concentrations (100, 10 and 1 µM) in triplicate. After a 96 h-incubation time cytotoxicity was measured on the Fluorolite 1000 (Dynatech, France) after a 4-h incubation time with Uptiblue® (Interchim, Montluçon, France). Inhibitory concentration 50 is a mean of triplicate values. A toxicity index was determined as follow: IC50 against MRC5/Geometric mean of the MICs against Candida spp.

Copyright and License information:  ©2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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