Beta-diversity was computed with the OTU table using UniFrac. Weighted UniFrac distances were used as inputs for PCoA based on the OTU table, purified to 5,000 sequences per sample using QIIME beta_diversity_through_plots.py command. Differences in community structures between before and after the start of solid foods were analyzed statistically based on the weighted Unifrac metric using the ‘adonis’ function with 999 permutations in the QIIME pipeline.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.