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CFA model of chronic inflammatory pain and drug treatment

SN Serena Notartomaso
PS Pamela Scarselli
GM Giada Mascio
FL Francesca Liberatore
EM Emanuela Mazzon
SM Santa Mammana
AG Agnese Gugliandolo
GC Giorgio Cruccu
VB Valeria Bruno
FN Ferdinando Nicoletti
GB Giuseppe Battaglia
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Inflammatory pain was induced by subcutaneous (s.c.) injections of 20 μl of Complete Freund’s Adjuvant (CFA, Sigma-Aldrich; 1 mg/ml) in the plantar surface of the right hind paw; control mice were injected s.c. with saline in the right hind paw. Groups of six to seven mice were treated i.p. daily for seven days with saline or NAC (25–100 mg/kg). Treatments started 10 min following CFA (or saline) injection. Mechanical pain thresholds were measured 1 h after the last injection. Mice were killed immediately after for protein measurements in the dorsal region of the lumbar spinal cord ipsilateral to CFA injections.

Copyright and License information: The Author(s) ©2020
Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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