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The HEK293 reporter cells (with TOPFlash reporter plasmid) or control cells (with FOPFlash reporter plasmid) were inoculated at 1000 cells/well and grown for 24 h. After 15 h incubation with Z-ajoene and/or Wnt3a-CM, luciferase activity in the cell lysates was measured by a luciferase assay system (Promega, Madison, WI, USA).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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