Transwell migration assay

THP-1 human monocyte chemotaxis assays were performed by using the HTS transwell plate system (5μm pore size, Corning). Conditioned medium was collected from Day 10 post-FACS hiPSC- and PGD-hESC-SCPs, and 2–5 week post FACS hiNC-Schwann cells, and 100uL of Schwann cell conditioned medium was placed in bottom well with or without anti-MCP1 (R&D). THP-1 monocytes were resuspended in Neurobasal medium (Gibco) + 1% FBS to a concentration of 2.5 million cells/mL, and 100ul was placed in upper wells. After 3 hrs incubation in 37 ˚C with 5% CO2, migrated THP-1 cells were counted. n defined by independent assays.