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Histopathologic analysis

KS Kotaro Shibagaki
CF Chika Fukuyama
HM Hironobu Mikami
DI Daisuke Izumi
NY Noritsugu Yamashita
TM Tsuyoshi Mishiro
NO Naoki Oshima
NI Norihisa Ishimura
SS Shuichi Sato
SI Shunji Ishihara
MN Mamiko Nagase
AA Asuka Araki
NI Noriyoshi Ishikawa
RM Riruke Maruyama
RK Ryoji Kushima
YK Yoshikazu Kinoshita
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Resected specimens were embedded in paraffin and mounted on slides, then subjected to hematoxylin and eosin staining and immunohistochemistry. Histologic diagnosis was determined based on discussions by expert pathologists (R.M., N.I., A.A., M.N.) in accordance with the Padova/Vienna classification, World Health organization (WHO) classification, and JCGC 8 15 16 17 . In addition, immunohistochemistry was performed to assess gastric mucin (MUC5AC for foveolar cells, MUC6 for mucous neck cells or pyloric gland cells) and intestinal mucin (MUC2 for goblet cells, CD10 for intestinal brush border) phenotype. Neoplastic proliferative activity was determined by calculating the Ki-67 labeling index and p53 protein expression was analyzed as a reference finding for TP53 gene mutations. Biomarkers used in this study were investigated using monoclonal antibodies to MUC5AC (Novocastra Laboratories Ltd., Newcastle, UK), MUC6 (Novocastra Laboratories), MUC2 (Novocastra Laboratories), CD10 (Dako, Copenhagen, Denmark), P53 (DO-7, Dako), and Ki-67 (MIB-1, Dako).

Copyright and License information:  ©2019
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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