For intracerebroventricular injections, FGF1 (3 µg, dissolved in sterile saline) or vehicle (saline) was injected over 10 s in a final volume of 4 µL using an injector with a 1-mm projection. For subcutaneous injections every other day, FGF1 was administered at a dose of 0.5 mg/kg body weight. All injections were done in the morning.
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