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Luciferase reporter assay

JK Ja Hyun Koo
SP Steven W. Plouffe
ZM Zhipeng Meng
DL Da-Hye Lee
DY Di Yang
DL Dae-Sik Lim
CW Cun-Yu Wang
KG Kun-Liang Guan
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Destabilized luciferase having PEST sequence (luc2P) were used for reporter assay. Cells were transfected with a pGL4.22-Luc reporter having a 2-kb region upstream of human FOS transcription start site. Luciferase activity was assayed using the Neolite reporter gene assay system (PerkinElmer Life Sciences) according to the manufacturer's instructions.

Copyright and License information:  ©2020-01-01
This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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