Four APTT, 1 dilute APTT (dAPTT), and 2 dRVVT screening reagents having high LA sensitivity were employed for the mixing tests in this study. The APTT reagents were Thrombocheck APTT‐SLA (SLA) (Sysmex Corporation, Kobe, Japan), Actin FSL (FSL) (Siemens Healthineers), APTT‐SP (SP) (Instrumentation Laboratory Company, Bedford, MA), and Cephen 2.5 LS (Hyphen BioMed, Neuville‐sur‐Oise, France), and the dAPTT reagent was PTT‐LA (PTT) (Diagnostica Stago UK, Theale, UK). The two dRVVT screening reagents were STACLOT DRVV Screen (dRVVT A) (Diagnostica Stago) and LA1 Screening reagent (dRVVT B) (Siemens Healthineers). These tests were performed in a CS‐2400 (Sysmex Corporation). The compositional characteristics of each reagent were previously described.12 All clotting time results were determined once, as imprecision is low with automated coagulometers.
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