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Supernatant was collected from mock- and VZV-infected qHPNCs (3 DPI), centrifuged, and flash frozen. The supernatant was thawed and added at 100% to uninfected qHPNCs or HCECs and replenished every 24 hours for 3 days. Coverslips were fixed and analyzed by immunofluorescence antibody assay (IFA) for adherens junction proteins.
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