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Proteins were extracted from cultured endothelial cells. The following primary antibodies were used: rabbit monoclonal anti-ICAM-1 (1:1000, ab53013, Abcam), rabbit polyclonal anti-ZO1 (1:1000, ab96587, Abcam), and rabbit monoclonal anti-phosphorylated NF-κB p65 at Ser536 (pp65, 1:1000, 3033S, Cell Signaling Technology). β-actin (1:5000, ab8226, Abcam) was used as the loading control. Western blots were performed at least three times in individual experiments.
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