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Untreated MDA-MB-231 cells were seeded on 96-well plates and incubated. When the cells reached 50% confluence, different concentrations (0, 2.5, 5, 10 mmol/l) of HP-β-CD were added to the medium. After 48 h, 20 µl MTT (5 mg/ml; cat. no. M8180; Beijing Solarbio Science & Technology Co., Ltd.) was added to each plate and after 4 h, the medium containing MTT was removed from 96-well plates and 200 µl DMSO was added to dissolve the formazan. Finally, the absorbance was measured at a wavelength of 490 nm; the experiment was performed in triplicate.
Copyright and License information: ©2019 Wu et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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