2.4. Hot plate

The hot plate test was conducted using a hot plate analgesia meter (COTM, Poland). The mice were placed on the plate, which was preheated to 52.5°C, and the time latency to the first sign of spinally mediated withdrawal reflexes (later described as a paw flinching) was measured. Moreover, we have measured the time latency to the first sign of complex behaviour (including licking/biting of the paw and/or jumping). A maximum exposure time was set at 30 s (cut‐off) to avoid tissue damage. Both types of responses were expressed as % MPE.