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The siRNAs targeting CTSD (NP599161) or control siRNA were transfected into NRVCs at 50nM using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific). Culture media were replaced 24hrs later by serum-free DMEM with the indicated glucose conditions. The Silencer® Pre-Designed siRNAs targeting CTSD (Cat. # AM16708 IDs: s191000, 191001, 191002) and the Silencer™ Select Negative Control siRNA (Cat. #4390846) were obtained from Thermo Fisher Scientific.
Copyright and License information: ©2019
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