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Western blots were performed as described previously (11) using primary anti-HMGB3 rabbit polyclonal antibody, ATM and p-ATM (Ser 1981), secondary anti-β actin rabbit polyclonal antibody (Abcam Biotechnology Company, Cambridge, UK), CHK2, p-CHK2 (Thr68), ATR, p-ATR (Ser428), and p-CHK1 (Ser317) (Cell Signaling Technology, Danvers, MA), and CHK1 (Santa Cruz Biotechnology, Dallas, TX).
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