Open field test

Locomotor activity and exploratory behavior were assessed using an open field test on 3 consecutive days at 7 and 25 d post dox. Caged mice were allowed to acclimate to the testing room for 30 min prior to test start. Meanwhile, 8 open field chambers (ENV-510; Med Associates, San Diego, CA, USA) were sprayed with 70% ethanol to clean the sensor surface and eliminate scent clues. Following acclimation, mice were placed into the chamber with minimal handling stress. The testing room was inaccessible during the 1-h testing session to eliminated external stimuli. Med Associates’ Activity Monitor 5 Monitoring Software was used to collect and analyze the data.