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293FT cells were cultured on glass slide, then fixed with 4% paraformaldehyde, and permeabilized with 0.5% Triton X-100. After blocking with 10% goat serum at room temperature for 60 min, specimens were incubated with primary antibody (anti-DDR2 antibody, 1:200 dilution; anti-HSP47 antibody, 1:100 dilution) and fluorochrome-conjugated secondary antibody (1:500 dilution) at 4 °C. Stained samples were imaged with an Eclipse 80i microscope (Nikon) or an Olympus FV1000 confocal microscope.
Copyright and License information: ©2019 Chen et al.
Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.
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