Mouse primary hepatocyte isolation and treatment

Mouse primary hepatocytes were isolated using an in situ perfusion collagenase IV method as described previously [²⁹]. Hepatocytes were cultured in 1:1 mixture of DMEM/Ham’s F-12 medium (Invitrogen, Carlsbad, CA) supplemented with 10% FBS. Primary hepatocytes were treated with 2 μM GW4064 (Sigma-aldrich) or 2 μM WAY-362450 with or without (+)-JQ1 (MedChem Express, Monmouth Junction, NJ) or C646 (MedChem Express) for several hours prior to gene expression analysis.