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Phenylalanine ammonia-lyase (PAL) activity (EC 4.3.1.5)
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PAL activity was measured according to the amount of phenylalanine conversion to cinnamic acid based on the method of D’cünha et al. (1996). The reaction mixture contained 100 mM Tris–HCl buffer (pH 8.5), 1 mM 2-mercaptoethanol, 50 mM l-Phenylalanine and enzyme extract. The reaction was terminated by the addition of 6 M HCl and absorbance of the supernatant was read at 290 nm.
Copyright and License information: Prof. H.S. Srivastava Foundation for Science and Society ©2019
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