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Adult female rat stomach and intestine tissues (snap-frozen after dissection by supplier) were obtained from Cell Biologics (Chicago, IL). Recombinant human P450 2S1 (Wu et al., 2006), human cytochrome b5 (Guengerich, 2005), and rat NADPH-P450 reductase (Hanna et al., 1998) were expressed in Escherichia coli and purified, as reported previously. Recombinant mouse P450 2s1, used in a few experiments, was tailored in the N terminus in a similar construct as the human version (Wu et al., 2006) and was expressed and purified in the same way.
Copyright and License information: ©2019 by The American Society for Pharmacology and Experimental Therapeutics
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