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Six to eight-week-old BALB/c mice were injected intraperitoneally (ip) with 106 infected RBCs with GFP-tagged P. berghei (ANKA). To evaluate parasitemia, tail smears were taken and stained with Giemsa (Baso Diagnostics Inc, Zhuhai, China), the number of parasites per 3000 RBCs were counted [33]. When the parasitemia reached 4–6%, the infected mice were used to feed mosquitoes that had been starved overnight; the mosquitoes were then maintained at 21 °C. Unengorged mosquitoes were removed 24 h post-blood meal. Midguts were dissected and infection intensity (oocyst number) were determined microscopically at day 8 post-infection.

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