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HEK293T cell culture

XC Xu Chen
YL Yaqiao Li
CW Chao Wang
YT Yinyan Tang
SM Sue-Ann Mok
RT Richard M. Tsai
JR Julio C. Rojas
AK Anna Karydas
BM Bruce L. Miller
AB Adam L. Boxer
JG Jason E. Gestwicki
MA Michelle Arkin
AC Ana Maria Cuervo
LG Li Gan
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HEK293T cells cultured in Dulbecco’s Modified Eagle’s medium (DMEM) with 10% fetal bovine serum were transfected with expression vectors encoding FLAG-tagged full-length hTau and myc-tagged p300, or empty vector, using lipofectamine 2000 (Life Technology). For mCherry-GFP-LC3 reporter, HEK293T cells were infected with lentivirus expressing mCherry-GFP-LC3, and a single clone of infected cells with moderate expression was isolated to establish the reporter line.

Copyright and License information: The Author(s). ©2020
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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