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Real-Time PCR

HW Haojia Wu
CL Chun-Fu Lai
MC Monica Chang-Panesso
BH Benjamin D. Humphreys
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cDNA was synthesized by reverse transcription from extracted RNA by iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). Expression of target genes was determined by real-time quantitative PCR using the iTaq SYBR Green Supermix and the iQ5 Multicolor Real-Time PCR Detection System (Bio-Rad). Glyceraldehyde-3-phosphate dehydrogenase was used as the internal control.

Copyright and License information:  ©2020 by the American Society of Nephrology

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