2.8. Differential Scanning Calorimetry experiments.

Differential Scanning Calorimetry (DSC) experiments were carried out using DSC Q2000 instrument (TA Instruments, New Castle, DE) in Tzero T4 heat flow calibration configuration. TA Instruments low volume hermetic alodined aluminum pellets were loaded with about 7 mg of aqueous MLV dispersion containing 10 w% of lipid. The instrument was programmed to cycle over a wide temperature range from 10 to 65 °C and zoomed in the range from 10 to 35 °C at the same rate of 1 °C/min for both the heating and cooling cycles. A total of five heating/cooling cycles were recorded for each of the samples. Experimental DSC curves were averaged out and the background drift was corrected using splines and a Matlab (MathWorks, Inc., Natick, MA) script.