Mouse peritonitis

Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Brigham and Women’s Hospital (2016N000145) and complied with institutional guidelines, the U.S. Department of Agriculture Animal Welfare Act, Public Health Service Policy on Humane Care and Use of Laboratory Animals, and the Institute for Laboratory Animal Research Guide for the Care and Use of Laboratory Animals (National Institutes of Health, Bethesda, MD, USA). Mice were fed ad libitum with PicoLab Rodent Diet 20-5053 (LabDiet, St. Louis, MO, USA). Mice were injected intraperitoneally with E. coli [10⁵ colony-forming units (CFUs), serotype O6:K2:H1] in sterile saline as previously described in Chiang et al. (36). Peritoneal exudates were harvested by lavage using 5 ml Dulbecco’s PBS (DPBS) without calcium or magnesium at 0 (naive), 4, 12, 24, 48, and 72 h. Spleens were also harvested from these mice at the same time intervals. The samples were then analyzed by LC-MS/MS and flow cytometry.