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YK Yasuharu Kakizaki
NM Naohiko Makino
TT Tomohiro Tozawa
TH Teiichiro Honda
AM Akiko Matsuda
YI Yushi Ikeda
MI Miho Ito
YS Yoshihiko Saito
WK Wataru Kimura
YU Yoshiyuki Ueno
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A Carl Zeiss Axio Observer D1 Inverted Microscope (Carl Zeiss Japan, Tokyo, Japan) was used for observation of the specimens at magnifications of ×50, ×100, or ×200, and 10 visual fields containing the enlarged pancreatic duct were selected arbitrarily from each case at a magnification of ×50 (2402161.63 μm2 for 1 field). The degree of fibrosis was calculated as the total fibrotic area in 10 selected fields divided by the total area of tissue stained with Masson trichrome, and the rate of immunopositivity was calculated as the total area positively stained in the same 10 selected fields divided by the total tissue area. AxioVision 4.7.1 (Carl Zeiss) was used for calculation of area.

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