2.8. Histopathological investigation

At the completion of the study, the mice were anaesthetized with chloroform and pieces of liver, kidney, and heart were removed so that they could be histopathologically examined. After the tissues were placed in 10% formalin solution and paraffin blocks were prepared, 5 μm sections were prepared from the tissues. To conduct histopathological investigations, hematoxylineosin staining was conducted on the sections and the lams were microscopically examined for possible lesions. To save time, the aforementioned measures were performed in control mice and mice receiving doses of 2500 and 5000 mg/kg of the extracts. If there were tissue changes in this stage, histopathological examinations were also performed in mice receiving lower doses of extracts [34].