2.5 |. Urinary 11-dehydro-thromboxane B2

Urine samples were stored at −70°C and shipped on dry ice to the Inflammatory Markers Laboratory for analysis. Urinary 11-dh TxB₂ (UTxB₂), a marker of aspirin response and inflammation, were measured using the AspirinWorks™ ELISA assay (Corgenix).¹⁴ Briefly, 100 μL of urine in assay buffer was incubated with mouse monoclonal antibody and 11-dh TxB₂-alkaline phosphatase tracer in a competitive fashion. UTxB₂ concentrations were determined by measuring color development at 405 nm with an Absorbance Microplate reader and expressed as pg/mL. Final results are reported as pg UTxB₂ per mg creatinine to normalize results for urine concentration.