2.7. Structure determination by circular dichroism

Circular dichroism spectra of the peptide (80 µM final concentration) were recorded with an optical path of 1 mm on a Jasco 810 (Japan) dichrograph in quartz suprasil cells (Hellma). Peptide signal was first recorded alone in 180 µL milliQ water. After addition of 20 µL of 1% (w/v) SDS (final concentration: 0.1% (w/v)) and homogenization of the solution a second measurement was performed. For each measurement, spectra of 3 accumulations were recorded between 190 and 260 nm (0.5 nm data pitch, 1 nm bandwidth) using the standard sensitivity.