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GCase activity assay
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The cells and mouse tissues were homogenized in 1% Na-taurocholate/1% Triton X-100 (Tc/Tx). GCase activity was determined fluorometrically with 4MU-Glc as substrate in 0.25% Tc/Tx diluted in 0.1 m citrate phosphate (CP) buffer (pH 5.6) (87). Mouse brains were homogenized in 1× PBS and incubated in 5 mm brain phosphatidylserine. The brain GCase activity was assayed in 0.1 m CP buffer (pH 5.6) with 4MU-Glc as substrate as described (88). Protein concentrations of cells and tissues were determined by BCA assay using BSA as standard.
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