2.14. Drug affinity responsive target stability (DARTS)

Lysates from SH‐SY5Y cells were incubated with or without andrographolide (1 μM) for 9 hr at room temperature as described by Lomenick et al. (2009). Lysates were then divided into seven portions for digestion with different concentrations of pronase E (1074330001, Sigma Aldrich) for 30 min at room temperature. Samples were boiled immediately after adding loading buffer to stop the digestion. Each sample was loaded onto 15% SDS‐PAGE for western blotting.