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Reduction of the disulfide bonds of A2V BsAb was performed using DTT. To this end, a 1 M DTT solution was prepared freshly and added to intact or IdeS-digested BsAb, to a final concentration of 20 mM. Reduction was thus performed for 1 h at 60°C.
Copyright and License information: ©2019 The Author(s). Published with license by Taylor & Francis Group, LLC.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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