qPCR

Total RNA was isolated from cells using the RNeasy RNA extraction kit (Qiagen), and cDNA was synthesized from 1 µg of total RNA using a iScript cDNA synthesis kit (Bio-Rad). Fluorescence real-time PCR analysis was conducted using iTaq Universal SYBR Green Supermix (Bio-Rad) and performed on the Bio-Rad C1000 thermal cycler (CFX-96 real-time PCR detection systems; Bio-Rad). The ribosomal protein large subunit P0 (RPLP0) mRNA level was used for normalization. The real-time PCR primers are: Cezanne 2, 5′-CACGAGCTGTAAACGGCTTCT-3′ and 5′-GCTTTCCGTAACACCAGGTCC-3′ and RPLP0, 5′-TTCATTGTGGGAGCAGAC-3′ and 5′-CAGCAGTTTCTCCAGAGC-3′.