Caspase3/7 activity assay

Cells were treated with vehicle or drugs in 96-well black-walled clear-bottom plates. After treatment, the CellTiter-Blue reagent (Promega) was added and plates were further incubated for 3 h before the cell viability was measured as mentioned above. The Caspase-Glo® 3/7 Reagent (Promega) was then added in each well and cells were further incubated for 1 h. Luminescence was then measured as per the manufacturer’s instructions. The caspase-3/7 activities were normalized to the cell viability and expressed as the percentages relative to the vehicle control of each cell line.