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Cell migration was assessed by a wound scratch assay. Briefly, 90% confluent SMCs were starved in low-serum media (0.1% FBS) overnight. Scratch wounds were created using a 2-mm-wide pipette tip. Cells were cultured in full-serum medium (10% FBS) with indicated treatment. After 24 h, the scratched area of cells was imaged by using Olympus IX73 imaging system. The average wounded area was quantified using Image-Pro Plus 6.0 software.
Copyright and License information: ©2019 the American Physiological Society
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